Journal: International Journal of Molecular Medicine

Article Title: Remimazolam alleviates myocardial ischemia/reperfusion injury and inflammation via inhibition of the NLRP3/IL-1β pathway in mice

doi: 10.3892/ijmm.2025.5498

Figure Lengend Snippet: Rema alleviates LPS-induced release and increased expression levels of IL-1β, IL-6 and TNF-α in cultured Raw264.7 cells. (A) Experimental protocol using cultured Raw264.7 macrophages. (B) LPS induced alterations in the release of IL-1β, IL-6 and TNF-α in Raw264.7 cells, while treatment with Rema inhibited these LPS-mediated changes (n=3). (C) LPS induced alterations in the gene expression levels of IL-1β, IL-6 and TNF-α in Raw264.7 cells, while treatment with Rema inhibited these effects (n=3). (D) DAPA inhibited the LPS-induced release of IL-1β, IL-6 and TNF-α in Raw264.7 cells, while treatment with Rema did not affect the alterations induced by DAPA (n=3). *** P<0.001. DAPA, dapansutrile; LPS, lipopolysaccharide; ns, not significant; Rema, remimazolam; RT-qPCR, reverse transcription-quantitative PCR.

Article Snippet: Cells were cultured in DMEM without FBS and pre-treated with 100 μ g/ml Rema (diluted in DMEM) for 20 min, and subsequently treated with lipopolysaccharide (LPS; 0.5 μ g/ml diluted in DMEM; cat. no. HY-D1056H, MedChemExpress) for 24 h in incubator at 37°C.

Techniques: Expressing, Cell Culture, Gene Expression, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: RpoS is a pleiotropic regulator of motility, biofilm formation, exoenzymes, siderophore and prodigiosin production, and trade-off during prolonged stationary phase in Serratia marcescens

doi: 10.1371/journal.pone.0232549

Figure Lengend Snippet: Strains and plasmids used in this study.

Article Snippet: S . marcescens 1912768R was isolated from rhizosphere soil in ginger fields and collected at the China Center for Type Culture Collection (strain number: CCTCC AB 2019405).

Techniques: Isolation, Mutagenesis, Cloning, Sequencing, Plasmid Preparation

Journal: PLoS ONE

Article Title: RpoS is a pleiotropic regulator of motility, biofilm formation, exoenzymes, siderophore and prodigiosin production, and trade-off during prolonged stationary phase in Serratia marcescens

doi: 10.1371/journal.pone.0232549

Figure Lengend Snippet: (A) Changes in the number of all viable strains and percentage of emerged white strains during the prolonged incubation of WT. (B) Frequency of de novo emergence of prodigiosin-defective mutants. R1-10 represent ten single colonies of WT 1912768R cultured independently under the same culture conditions. (C) Representative images of the WT strain 1912768R and the two mutants. (D) Quantification of prodigiosin production in LB medium. *, P < 0.05; ***, P< 0.001.

Article Snippet: S . marcescens 1912768R was isolated from rhizosphere soil in ginger fields and collected at the China Center for Type Culture Collection (strain number: CCTCC AB 2019405).

Techniques: Incubation, Cell Culture

Journal: PLoS ONE

Article Title: RpoS is a pleiotropic regulator of motility, biofilm formation, exoenzymes, siderophore and prodigiosin production, and trade-off during prolonged stationary phase in Serratia marcescens

doi: 10.1371/journal.pone.0232549

Figure Lengend Snippet: Multiple sequence alignment of RpoS from S . marcescens 1912768R, Serratia sp. YD25, S . plymuthica AS9, E . coli str . K-12 substr. MG1655 and Serratia sp. ATCC 39006. The alpha helix, random coil and beta turn are represented as α, η and T, respectively. Conserved sequences are indicated with a red box, and similar sequences are indicated using a colored background.

Article Snippet: S . marcescens 1912768R was isolated from rhizosphere soil in ginger fields and collected at the China Center for Type Culture Collection (strain number: CCTCC AB 2019405).

Techniques: Sequencing

Journal: PLoS ONE

Article Title: RpoS is a pleiotropic regulator of motility, biofilm formation, exoenzymes, siderophore and prodigiosin production, and trade-off during prolonged stationary phase in Serratia marcescens

doi: 10.1371/journal.pone.0232549

Figure Lengend Snippet: (A) The rpoS mutant colony and color phenotypes were complemented by rpoS from S . marcescens 1912768R. (B) qRT-PCR analysis revealed reduced expression from the prodigiosin biosynthetic locus. (C-D) Growth curves of the WT strain 1912768R and the Δ rpoS mutant in LB or M9 minimal medium supplemented with 0.2% glucose. (E) Complementation of Δ rpoS mutant defects by rpoS genes from Serratia sp. strains with different pig cluster types and E . coli .

Article Snippet: S . marcescens 1912768R was isolated from rhizosphere soil in ginger fields and collected at the China Center for Type Culture Collection (strain number: CCTCC AB 2019405).

Techniques: Mutagenesis, Quantitative RT-PCR, Expressing

Journal: PLoS ONE

Article Title: RpoS is a pleiotropic regulator of motility, biofilm formation, exoenzymes, siderophore and prodigiosin production, and trade-off during prolonged stationary phase in Serratia marcescens

doi: 10.1371/journal.pone.0232549

Figure Lengend Snippet: The WT strain 1912768R and in-frame deletion Δ rpoS mutant were exposed to 3 M NaCl solution (A), 175 mM acetic acid (B), 50°C (C) and 120 mM H 2 O 2 (D). The survival percentage was obtained by dividing the surviving population by the initial population, which corresponded to 100%. Data are mean and SD of three independent experiments.

Article Snippet: S . marcescens 1912768R was isolated from rhizosphere soil in ginger fields and collected at the China Center for Type Culture Collection (strain number: CCTCC AB 2019405).

Techniques: Mutagenesis

Journal: PLoS ONE

Article Title: RpoS is a pleiotropic regulator of motility, biofilm formation, exoenzymes, siderophore and prodigiosin production, and trade-off during prolonged stationary phase in Serratia marcescens

doi: 10.1371/journal.pone.0232549

Figure Lengend Snippet: (A-B) Growth of the 1912768R and Δ rpoS mutant strains on 96-well plates containing SDS or Cm. (C) Change in transcript levels of luxS and porin-related genes (n = 4). (D-E) Effect of rpoS on the fitness of S . marcescens . S . marcescens 1912768R with theΔ rpoS mutant at a 1:1 ratio and grown for 24 and 48 h in LB or M9 medium. Samples were removed, diluted and plated on LBA plates for CFU determination. The number of 1912768R and Δ rpoS mutant colonies was verified by the different color of the colonies. Mean and SD are shown. *, P < 0.05; **, P < 0.01.

Article Snippet: S . marcescens 1912768R was isolated from rhizosphere soil in ginger fields and collected at the China Center for Type Culture Collection (strain number: CCTCC AB 2019405).

Techniques: Mutagenesis

Journal: Heliyon

Article Title: Characterization of a rare bla VIM-4 metallo-β-lactamase-producing Serratia marcescens clinical isolate in Hungary

doi: 10.1016/j.heliyon.2020.e04231

Figure Lengend Snippet: MIC values of the tested S. marcescens strain.

Article Snippet: Ghait et al. was first to report the detection of MBL-producing S. marcescens isolates (including strains harbouring the bla IMP-4 [42.5%] and bla VIM-2 [37.5%] genes) from inpatients with bacteremias in Egypt [ ].

Techniques:

Journal: Parasites & Vectors

Article Title: Bacterial feeding, Leishmania infection and distinct infection routes induce differential defensin expression in Lutzomyia longipalpis

doi: 10.1186/1756-3305-6-12

Figure Lengend Snippet: Defensin expression analysis in L . longipalpis fed with bacteria or orally infected with Leishmania. Female L . Longipalpis fed on suspensions of ( A ) E . coli ; ( B ) Ochrobactrum sp.; ( C ) S . marcescens ; ( D ) P . agglomerans ; ( E ) M . luteus , were collected at 24, 48, 72 and 96 h after feeding (gray bars). Insects fed on sterile sucrose solution were used as control (white bars). ( F ) Female L . longipalpis fed on blood seeded with L . mexicana were collected at 24, 48, 72, 96, and 144 h after infection (black bars). Insects fed on blood were used as control (dark gray bars). The relative defensin gene expression was normalized against the housekeeping internal control gene 60S-rRNA. Bar charts represent mean ± SEM of 3 pools of 3 insects. Asterisks represent statistical significance at P < 0.05.

Article Snippet: Escherichia coli (K12 RM148), Micrococcus luteus (A270), Ochrobactrum sp. (OM1,198 Jacobina colony isolate), Pantoea agglomerans (NCIMB11392), and Serratia marcescens (NCIMB 1377) were inoculated on Luria-Bertani (LB) agar plates and incubated overnight for 24 hours at 37°C.

Techniques: Expressing, Bacteria, Infection, Sterility, Control, Gene Expression